DNA-based Onychomycosis Detection Correlates Better to Histopathology than does Fungal Culture

2020-09-16T13:35:20-04:00

Onychomycosis is a prevalent disease of the nail. Traditional methods for diagnosis include direct microscopy with potassium hydroxide (KOH microscopy) and fungal culture. OUR PATHOLOGY SERVICES | PHYSICIAN RESOURCES | INSTRUCTIONAL VIDEOS | ORDER LAB SUPPLIES | FIND AN ACCOUNT MANAGER Traditional methods for diagnosis include direct microscopy with potassium hydroxide (KOH microscopy) and fungal culture. Other techniques using histochemical staining have higher sensitivity, but cannot identify genus or species of the infecting agent. PCR assays are sensitive, specific, and capable of genus and species level identification. We describe a real-time PCR assay for 15 different fungi that [...]